4-氯-2-氧代-3

摘　要：报道了4-氯-2-氧代-3(2H)-苯并噻唑乙酸乙酯除草剂及相关化合物的反相高效液相色谱分析方法。基线分离了4-氯-2-氧代-3(2H)-苯并噻唑乙酸乙酯及相关化合物5个组分，定量测定了4-氯-2-氧代-3(2H)-苯并噻唑乙酸乙酯和2-羟基-4-氯苯并噻唑，方法的相对标准偏差RSD分别为1.68%和1.03%，平均回收率分别为101.0%和100.2%，检测限分别为10.2ng和4.6ng。

关键词：4-氯-2-氧代-3(2H)-苯并噻唑乙酸乙酯，除草剂，反相高效液相色谱
分类号：O65　文献标识码：A

Analysis of 4-Chloro-2-oxo-3(2H)-benzothiazoleacetic Ethyl Ester and Related Compounds by High-Performance Liquid Chromatography

Shao Tao（Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041）　
Long Yuande（Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041）　
Huang Tianbao（Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041）　

Abstract：A reversed-phase high performance liquid chromatographic method for analyzing 4-chloro-2-oxo-3(2H)-benzothiazoleacetic ethyl ester (COBTAZAE) as a herbicide is described. COBTAZAE and the related compounds, such as 4-chloro-2-oxo-3(2H)-benzothiazoleacetic acid (COBTAZAA), 2- amino-4-chlorobenzothiazole (ACBTAZ), 2-hydroxyl-4-chlorobenzothiazole (HCBTAZ), and 2,4-dichlorobenzothiazole (DCBTAZ) were well separated through a Kromasil-C18(200x4.6mmID, 5μm) by using CH₃OH/H₂O/CH₃COOH(60/40/1, V/V) as mobile phase and detection at 254　nm.The good linear relationships of the injected amount versus peak area were observed with the correlation coefficients of 0.9995 and 0.9991 for COBTAZAE and HCBTAZ, respectively. The relative standard deviation, average recovery and detection limit for determining COBTAZAE were found to be 1.7%, 101.1% and 10.2 ng, respectively, and that for HCBTAZ 1.0%, 100.2% and 4.6 ng, respectively.
Keywords：4-Chloro-2-oxo-3(2H)-benzothiazoleacetic ethyl ester, herbicide, reversed phase high performance liquid chromatography

参考文献：

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收稿日期：2000年2月21日

修稿日期：2000年7月25日

出版日期：2001年1月20日