相关文摘：色谱指纹图谱定性相似度和定量相似度的比较研究

ABSTRACT: AIM To exploit the differences between the qualitative similarity and the quantitative similarity of Traditional Chinese Medicine chromatographic fingerprints, the quantitative similarity calculated by vector shadow C%, approximate quantitative similarity R%, quantitative similarity P%, etc. were firstly proposed to disclose the quantitative information characteristics of Traditional Chinese Medicine fingerprints. METHODS The HPLC fingerprints of both the standard Fructus Gardeniae and the ten batches of Fructus Gardeniae produced in different places were evaluated by the new parameters to obtain good results. The standard fingerprint contained 35 peaks while geniposide was selected as the reference peak. RESULTS The HPLCFP had good precision and reproducibility with the RSD of the relative migration time less than 1.5% and the RSD of the relative peak area within 5%. The qualitative similarity and quantitative similarity between each crude drug and the contrasted fingerprint were quantitatively calculated, the values of C%, P%, etc., were applied in the quality control practice, which had less errors. What is more, this method could be used for the overall quality control of Fructus Gardeniae and especially suits for qualitatively and quantitatively evaluated the chromatographic fingerprints both in chemical constituent distribution and in contents. CONCLUSIONS The quantitative parameters such as C% and P% can be used to objectively, authentically and thoroughly display the content information characteristics. When they combined with the qualitative similarity, it was the good method to evaluate the Traditional Chinese Medicine chromatographic fingerprints.