固相萃取富集高效液相色谱法测定苯氧乙酸和2,4-二氯苯氧乙酸

摘　要：建立了以固相萃取技术进行富集，高效液相色谱法进行分离和检测苯氧乙酸和2，4-二氯苯氧乙酸的方法。环境水中的苯氧乙酸和2,4-二氯苯氧乙酸用Sep-PakC₁₈Cartridge进行固相萃取。液相色谱的条件是：Shim-PackCLCODS柱为分析柱；甲醇-水(9:1,V/V)为流动相；流速为1mL/min；在UV-275nm波长下进行检测。本法具有良好的灵敏度和重现性。

关键词：高效液相色谱，固相萃取，苯氧乙酸，2,4-二氯苯氧乙酸，除草剂

分类号：O65　文献标识码：A

Determination of Phenoxyacetic Acid and 2,4-Dichlorophenoxyacetic Acid Using Solid-phase Extraction and High Performance Liquid Chromatography

Li Jinchang（Center of Analysis and Test,Northeast Normal University,Changchun 130024）　
Wang Lu（Center of Analysis and Test,Northeast Normal University,Changchun 130024）　
Han Mingyou（Center of Analysis and Test,Northeast Normal University,Changchun 130024）　
Bao Ming（Center of Analysis and Test,Northeast Normal University,Changchun 130024）　
Wang Guang（Center of Analysis and Test,Northeast Normal University,Changchun 130024）　
Wang Yuanhong（Center of Analysis and Test,Northeast Normal University,Changchun 130024）　

Abstract：A high performance liquid chromatographic method for the determination of phenoxyacetic acid and 2,4-dichlorophroxyacetic acid in environnmental waters was described.Phenoxyacetic acid and 2, 4-dichioropheroxyacetic acid in sample were enriched on Sep-Pak C₁₈ column,and methanol was employed as elutent.The shim-pack CLC ODS (150mm×6.0mm i.d.) column was used as separation column at 40°C and eluted with methanol-water (9:1,V/V)(pH 3.0) at flow rate of 1.0mL/min,the detection wavelength was 275 nm.The recoveries of the two compounds were＞98%.The detection limit was 0.04mg/L.
Keywords：High performance liquid chromatography,solid-phase extraction,phenoxyacetic acid,2,4-dichioropheroxyacetic acid, herbicide

参考文献：

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收稿日期：2000年7月12日

修稿日期：2001年1月18日

出版日期：2001年5月20日