猪血浆中鹿蹄草素的HPLC测定及药代动力学研究

摘　要：用高效液相色谱法测定血浆中鹿蹄草素的浓度。方法：采用YWG-C18色谱分析柱(4.6mm×250mm,5μm),WatersTM486可变波长紫外检测器，甲醇-水(40∶60)为流动相，香草醛为内标。血液样品用乙醚提取纯化后进样，在220nm波长处检测。结果：鹿蹄草素线性范围为1.0～100.0mg^.L^-1(r=0.9953)，最低检测量0.1μg，最低检出浓度5.0mg^.L^-1，平均方法回收率98.36%，精密度考察鹿蹄草素日内、日间RSD均小于4%。结论：本方法专属性高，快速，准确，重复性好。

关键词：鹿蹄草素　血药浓度　HPLC　药代动力学

分类号：R927　文献标识码：A

Studies on HPLC Determination and Pharmacokinetics of Pyroline in Pig Plasma

LI Jian-yong（Lanzhou Institute of Animal & Veterinary Pharmaceutics Science,Chinese Academy of Agricultural Sciences,Lanzhou 730050）　
ZHAO Rong-cai（Lanzhou Institute of Animal & Veterinary Pharmaceutics Science,Chinese Academy of Agricultural Sciences,Lanzhou 730050）　
ZHANG Ji-yu（Lanzhou Institute of Animal & Veterinary Pharmaceutics Science,Chinese Academy of Agricultural Sciences,Lanzhou 730050）　
XU Zhong-zan（Lanzhou Institute of Animal & Veterinary Pharmaceutics Science,Chinese Academy of Agricultural Sciences,Lanzhou 730050）　
LI Jin-shan（Lanzhou Institute of Animal & Veterinary Pharmaceutics Science,Chinese Academy of Agricultural Sciences,Lanzhou 730050）　

Abstract：Objective：A HPLC method is described for the analysis of pyroline in pig plasma.Methods:The YWG-C18 column (4.6 mm×250 mm,5 μm) was used,The mobile phase consisted of methanol-water (40∶60),at a flow rate of 0.7 mL^.min^-1.Vanillin was adopted as the internal standard at UV absorption of 220 nm.Results:The ranges of pyroline calibration curves were 1.0～100.0 mg^.L^-1(r=0.995 3). Detection limits were 0.1 μg and the lowest detectable concentration of pyroline was 5.0 mg^.L-1,while the average recovery of the method was 98.36%.The RSD of intra-day and inter-day assays was all less than 4%.Conclusion:The method is quick,precise and good repeat.

Keywords：pyroline,drug concentration,plasma,HPLC,pharmacokinetic

参考文献：

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收稿日期：2000年3月21日

出版日期：2001年3月