胶束电动毛细管色谱分离氨基酸和磷酸化氨基酸

摘　要：本文报道了胶束电动毛细管色谱分离、汞灯诱导荧光电荷耦合器件检测分析氨基酸和磷酸化氨基酸的4-氟-7-硝基苯-2-?-1，3-丫唑衍生物。研究表明，在pH9.35的10mmol/L硼砂和10mmol/L十二烷基硫酸钠的电泳缓冲介质中，5种氨基酸和3种磷酸化氨基酸在10min内完全分离，检测灵敏度为1.21×10^-8～5.21×10^-8mol/L，分离效率达7.3×10⁵～3.0×10⁵/m理论塔板数，结果令人满意。

关键词：胶束电动毛细管色谱；氨基酸；磷酸化氨基酸；荧光检测

分类号：O657.8；Q517　文献标识码：A
文章编号：1006-6144(2001)02-0102-04

Separation of Amino Acids and Phosphoamino Acids as Their Derivatives by Micellar Electrokinetic Capillary Chromatography

LIU Bi-feng（Key Laboratory of MOE for Plant Developmental Biology, Wuhan University, Wuhan, 430072）　
ENG Yong-hui（Key Laboratory of MOE for Plant Developmental Biology, Wuhan University, Wuhan, 430072）　
LU Ying-tang（Key Laboratory of MOE for Plant Developmental Biology, Wuhan University, Wuhan, 430072）　

Abstract：The separation of amino acids and phosphoamino acids as their 4-fluoro-7-nitroben-2-oxa-1,3-diazple fluorescent derivatives was achieved by micellar electrokinetic capillary chromatography with lamp induced fluorescence-cooled charged coupled device detection. Some parameters such as precapillary derivatization conditions and separation conditions were discussed and optimized. With a buffer of pH 9.35 10 mmol/L borate containing 10 mmol/L SDS, the 5 amino acids and 3 phosphoamino acids were separated in 10 min. The detection limits were 1.21×10^-8～5.21×10^-8 mol/L with separation efficiency of 7.3×10⁵～3.0×10⁵/m.
Keywords：Micellar electrokinetic capillary chromatography; Amino acids; Phosphoamino acids; Fluorescence detection

参考文献：

［1］孙大业,郭艳林,马力耕.细胞信号转导[M].北京：科学出版社,1998：143.
［2］Crowder M W, Muman A Q, Haddadian F et al. Anal. Chim. Acta[J], 1999, 384: 127.
［3］张乐.武汉大学硕士学位论文[D],1998.
［4］Lalljie S P, Sandra P. Chromatographia[J], 1995, 40: 519.
［5］Mattusch J, Dittrich K. J. Chromatogr. [J], 1994, 680: 279.
［6］Haugland R P. Handbook of Fluorescent Probes and Research Chemicals{sixth edition)[M]. Molecular Probes, Iac., 1996:40.

收稿日期：2000年9月13日

出版日期：2001年4月20日