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Abstract：The analytical method of chlorogenic acid in sunflower cake by th
in-layer chromatography(TLC) was developed. With 36% acetic acid as m obile
phase and on the polyamide film plate, the Rf value of chlorogenic a
cid was 0.61. The calibration curve was linear in the range of 0.05 μg-0.6 μg of chlorogenic acid with
the detection limit of 0.025 μg and the average r ecovery of 97.53%. The
inter-plate accuracy of chlorogenic acid was 2.57%. Wi th 70% ethyl alcohol as
extracting solvent and 30 min of extraction time, the am ount of chlorogenic
acid in the extract, the residue amount of chlorogenic acid in the sunfl ower
cake and the extraction rate were determined and calculated by applying thi s
method. The optimal time of extraction was
found. |
| 作者简介：朱 玉(1965-)，女，工程师，电话：(037 1)7763390，E-mail:dcx@mail.zzu.edu.cn。
作者单位：朱玉（郑州大学分析测试中心， 河南 郑州 450052） 张书胜（郑州大学分析测试中心， 河南 郑州 450052） 张西林（郑州大学分析测试中心， 河南 郑州 450052） 陈洁（郑州粮食学院食品 工程系， 河南 郑州 450052） 参考文献： ［1］ Sosulski F. J Am Oil Chem Soc, 1979,56(8):711-715 |
原载于《色谱》2001 Vol.19 No.1 P.82-84
http://periodical.wanfangdata.com.cn/periodical/sp/sp2001/0101/0101ml.htm