HPLC法测定舒脑宁长效片中二氢麦角毒碱甲磺酸盐的含量

摘　要：建立一种用HPLC测定舒脑宁长效片中二氢麦角毒碱甲磺酸盐含量的方法。方法：用C₁₈柱，甲醇-0.005mol^.L^-1磷酸二氢铵溶液(用磷酸调pH至4)(85∶15)为流动相；检测波长为280nm。结果：该方法回收率为99.1%，RSD=0.5%(n=7)。结论：该方法准确，简便，快捷，重现性好及应用广泛。

关键词：二氢麦角毒碱甲磺酸盐　舒脑宁长效片　高效液相色谱法

分类号：R927　文献标识码：A

Determination of Dihydroergotoxine Mesylate in Ischelium Retard by HPLC

LIU Li-fang（Guangzhou Municipal lnstitute for Drug Control,Guangzhou 510160）　
ZHAO Xue-mei（Guangzhou Municipal lnstitute for Drug Control,Guangzhou 510160）　
WEI Lian（Guangzhou Municipal lnstitute for Drug Control,Guangzhou 510160）　

Abstract：Objective：To develop an HPLC method for the determination of dihydroergotoxine mesylate in Ischelium Retard. Methods:C₁₈ column was used as the stationary phase,at a flow rate of 1 mL^.min^-1,MeOH-0.005 mol^.L^-1 (NH₄)₂HPO₄(pH adjusted to 4 with phosphoric acid)(85∶15) was used as the mobile phase.The detection wavelength was 280 nm.Results:The recovery percentage of the method was 99.1% with RSD 0.5%(n=7).Conclusion:The method is simple,rapid and accurate.

Keywords：dihydroergotoxine mesylate,Ischelium Retard,HPLC

参考文献：

［1］USP. ⅩⅩⅢ. 603
［2］LUO Ming-sheng(罗明生),GAO Tian-hui(高天惠).The Complete Collection of Pharmaceutical Excipients(药剂辅料大全).Sichuan(四川)：Technology and Science Press(科学技术出版社),1993.383(in Chinese)
［3］Poly Firm Limited(庞权有限公司).ISCHELIUM Technical Data(舒脑宁长效片检验资料)

收稿日期：2000年2月28日

出版日期：2001年3月31日