HPLC法快速测定5-氟尿嘧啶血药浓度

摘　要：目的：建立反相高效液相色谱法测定人血清中5-氟尿嘧啶浓度。方法：血清样品用乙酸乙酯提取，水浴氮气吹干，残留物用水溶解后进样。色谱柱为C₁₈柱(250mm×4.6mm)，水为流动相，流速1.0mL·min^-1，紫外检测波长273nm，桂皮酸为内标。结果：本法最低检测浓度为0.05μg·mL^-1，线性范围为1.0～50.0μg·mL^-1，日内RSD为2.7%～4.1%，日间RSD为3.8%～4.7(n=4)。结论：该法适用于5-氟尿嘧啶的药代动力学研究及临床血药浓度检测。试验结果表明，该方法经济、简便、快速、灵敏度高、重现性好。

关键词：高效液相色谱　5-氟尿嘧啶　血药浓度

分类号：R927　文献标识码：A

A Simple and Rapid HPLC Determination of 5-Fluorouracil in Human Serum

LIU Chuan-hua（Laboratory of Molecular Biology of Medical College,Shandong University,Jinan 250012）　
LIU Shi-lian（Laboratory of Molecular Biology of Medical College,Shandong University,Jinan 250012）　
BIAN Ji-feng（Laboratory of Molecular Biology of Medical College,Shandong University,Jinan 250012）　
GENG Zhao（Laboratory of Molecular Biology of Medical College,Shandong University,Jinan 250012）　
LU Yi（Laboratory of Molecular Biology of Medical College,Shandong University,Jinan 250012）　
HU Hai-yan（Laboratory of Molecular Biology of Medical College,Shandong University,Jinan 250012）　

Abstract：Objective：To develop a simple and rapid HPLC method for the determination of 5-fluorouracil (5-Fu)concentration in human serum.Methods:The serum samples were extracted with ethyl acetate.The solvent was evaporated to dryness under a gentle stream of N2 at 40　℃ in a water bath.The analysis involved a C18 column as stationary phase and distilled water as mobile phase.The flow rate was 1.0 mL·min^-1,UV detection wavelength was at 273 nm.Cinnamic acid was used as the internal standard.Results:The minimal detectable serum drug concentration was 0.05 μg·mL^-1.The calibration curve was linear over a range from 1.0 μg·mL^-1 to 50.0 μg·mL^-1 with a correlation coefficient of 0.999 4.Intra-day and inter-day RSD were 2.7%～4.1% and 3.8%～4.7%(n=4) respectively.Conclusion:The method is economic ,simple,rapid,sensitive and accurate.

Keywords：HPLC,5-fluorouracil,serum drug concentration

参考文献：

［1］Speyer J L.A study on 5-Fluorouracil in advanced malignant tumors.Semin Oncol,1985,12(3):23
［2］QING San-hua(卿三华),ZHOU Xi-geng(周锡庚),XU Jun-chang(许俊昌),et al.Studies on 5-fluorouracil in human serum,biological fluid and tissue by HPLC(高效液相色谱法测定血液、体腔和组织中5-Fu浓度的研究).Tumor(肿瘤),1990,10(4)：168(in Chinese)
［3］WANG Dong-mei(王冬梅),XIE Jing-wen(谢景文).Determination of 5-Fluorouracil concentration in human serum by HPLC(高效液相色谱法测定人血清中5-氟尿嘧啶浓度).Chin J Chromatogr(色谱),1997,15(2)：164(in Chinese)

收稿日期：2000年3月3日

出版日期：2001年3月31日