HPLC测定中肝合剂中芍药苷及栀子苷的含量

摘　要：目的　建立一种反相高效液相色谱法测定中肝合剂中芍药苷和栀子苷含量的方法。方法　用C18ODS柱，以乙腈-0.1%磷酸为流动相，检测波长230nm。结果　平均回收率：芍药苷98.78%(RSD＝2.43%,n＝5)；栀子苷99.60%(RSD＝1.88%，n＝5)。结论　该方法简便，快捷，准确。

关键词：芍药苷；栀子苷；高效液相色谱法

分类号：R917；R927.2　文献标识码：A
文章编号：1001-2494(2001)01-0048-02

Determination of paeoniflorin and geniposide in Zhonggan oral liquid by HPLC

LU Bai-zhen（Zhejiang Tumor Hospital,Hangzhou 310022,China）　
LI Xin-hong（Institute for Drug Control of Hangzhou,Hangzhou 310014,China）　
YANG Jiang-feng（Institute for Drug Control of Hangzhou,Hangzhou 310014,China）　

Abstract：OBJECTIVE　A RP-HPLC method was establised for the determination of paeoniflorin and geniposide in Zhonggan oral liquid.METHODS　The c18 column was used.The mobile phese was consisted of CH3CN-0.1% H3PO4(15∶85).The detection wavelength was at 230 nm.RESULTS　The average recoveries were 98.78%(RSD=2.43%,n=5)for paeoniflorin and 99.60%(RSD=1.88%,n=5)for geniposide,respectively.CONCLUSION　The method was simple,rapid and accurate.

Keywords：paeoniflorin;geniposide;HPLC

收稿日期：1999年6月10日

出版日期：2001年1月8日