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摘 要:许多分析问题的解决需要得到比一维色谱技术能提供的更高的分辨率。分离能力可通过使用多种分离技术或机理的组合来增强。此时,样品被分散在不同的时间维,最终的分辨率强烈地依赖于这些维间分离特性的差异。当它们之间没有关联,也即相互间正交时,系统可获得最高的分辨率。全二维气相色谱(GC×GC)提供了一个真正的正交分离系统。它把分离机理不同而又互相独立的两支色谱柱以串联方式结合组成二维气相色谱。在这两支色谱柱之间装有的一个调制器起捕集再传送的作用。全二维色谱的峰容量为组成它的两支色谱柱各自峰容量的乘积。介绍了GC×GC的技术和仪器,比较了现阶段GC×GC系统的3种设计。列举了在石化和环保领域GC×GC的许多应用实例。简单地讨论了GC×GC将来的发展前景。
Abstract:Many
analytical problems require more resolution than the conventional single
column chromatographic technique can provide. In
such cases the separation power can be enhanced by using more than one
separation technique or mechanism. The
sample is then dispersed in different time dimensions.
The resolution obtained
depends strongly on the difference between these dimensions.
The highest resolution is
gained when there is no correlation between the separations, the
dimensions being orthogonal to each other. Comprehensive
two-dimensional gas chromatography (GC×GC) provides a true orthogonal
separation system in which a modulator serially couples two columns
containing dissimilar stationary phases. It
focuses and subsequently reinjects components eluting from the first
column into the second one. The
system generates a peak capacity that is approximately equal to the
product of the peak capacities of the two individual separation systems.
In this paper, technique
and instrumental considerations of GC×GC are discussed.
The three designs of
contemporary GC×GC systems are presented and compared.
A number of typical
applications on complex samples such as petroleum products and
environmental pollutants are also cited. Finally,
the future perspectives of GC×GC are simply discussed. |
作者简介:许国旺(1963-),男,研究员,博士生导师,电话:(0411)3693403,E-mail:dicp402@mail.dlptt.ln.cn。 作者单位:许国旺(中国科学院大连化学物理研究所国家色谱研究分析中心, 辽宁大连 116011) 叶芬(中国科学院大连化学物理研究所国家色谱研究分析中心, 辽宁大连 116011) 孔宏伟(中国科学院大连化学物理研究所国家色谱研究分析中心, 辽宁大连 116011) 路鑫(中国科学院大连化学物理研究所国家色谱研究分析中心, 辽宁大连 116011) 赵欣捷(中国科学院大连化学物理研究所国家色谱研究分析中心, 辽宁大连 116011) 参考文献: [1]Schomburg G. J
Chromatogr A, 1995,703:309-325 |
原载于《色谱》2001 Vol.19 No.2
P.132-136
http://periodical.wanfangdata.com.cn/periodical/sp/sp2001/0101/0101ml.htm