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Abstract:A method for the determination of main composition in degraded products
of penicillin, 6-aminopenicillanic acid (6-APA),penicillin G ka lium(PGK),
phenylacetic acid(PAA), and the by-product benzylpenicilloic acid(BP A) by HPLC
was studied. Separation conditions were as follows: Spherisorb C18
column, 250 mm×4.6 mm i.d., 10 μm; mobile phase: V(methanol)∶V (0.004 mol/L
KH2PO4 buffer, pH 4.5)=50∶50; flow rate: 1 mL/min; detector: UV 230 nm. The
method is effective, quick, accurate and reproducible. The satis factory results
show that this new method has certain practical values as an approach of quality
control. |
基金项目:国家“九.五”重点攻关课题(96-0C02-0201) 作者简介:赵静玫(1957-),女,工程师,电话:(0411)4671991-859,E-mail:zhaomei@dicp.ac.cn. 作者单位:赵静玫(中国科学院大连化学物理研究所, 辽宁 大连 116023) 都绛瑛(中国科学院大连化学物理研究所, 辽宁 大连 116023) 张铭俊(中国科学院大连化学物理研究所, 辽宁 大连 116023) 参考文献: [1] WU Run-ling, CAI Shun-xin. Chinese Journal of Anti-biotics,
1996, 21(3):234-236 |
原载于《色谱》2001 Vol.19 No.1 P.88-90
http://periodical.wanfangdata.com.cn/periodical/sp/sp2001/0101/0101ml.htm