青霉素裂解液的高效液相色谱分析

Abstract：A method for the determination of main composition in degraded products of penicillin, 6-aminopenicillanic acid (6-APA),penicillin G ka lium(PGK), phenylacetic acid(PAA), and the by-product benzylpenicilloic acid(BP A) by HPLC was studied. Separation conditions were as follows: Spherisorb C₁₈ column, 250 mm×4.6 mm i.d., 10 μm; mobile phase: V(methanol)∶V (0.004 mol/L KH₂PO₄ buffer, pH 4.5)=50∶50; flow rate: 1 mL/min; detector: UV 230 nm. The method is effective, quick, accurate and reproducible. The satis factory results show that this new method has certain practical values as an approach of quality control.
Keywords：high performance liquid chromatography; 6-aminopenicillanic acid; penicillin G kalium; phenylacetic acid; benzylpenicilloic acid; penicillin acylase; penicillase