2.3 毛细管电泳法(Capillary Electrophoresis,CE)
毛细管电泳作为迄今分辨率最高的分离技术,应用日趋广泛。Chan等[28]首次将高效毛细管电泳(HPCE)引入紫杉醇的分析,采用的是胶束电动色谱(MEKC)分离模式。Hempel等[29]则成功地将MEKC用于生物体液中紫杉醇的定量分析,检测中使用紫外检测器(228nm),检测限可达20ng.mL-1,线性范围为50~5000ng.mL-1,显示了良好的应用前景。 2.4 细胞生物学方法(Bioassay)
紫杉醇是一种作用机理独特的新型抗癌药物,它通过诱导微管蛋白聚合,并阻止其解聚,从而抑制细胞分裂,达到抗肿瘤目的。根据这一性质,Shuler等[30]进行了利用紫杉醇依赖性细胞系来筛选紫杉烷类化合物的研究,但该方法缺少专一性,限制了其应用。 3 结语
综上所述,对紫杉醇的提取分离和分析检测的研究已取得了长足的进展,一些新的技术和手段不断应用于这一领域,并显示出诸多优势和良好前景。但从目前研究情况看,反相HPLC仍发挥着主要作用。分析植物材料主要用苯基柱,而C18柱则多用于生物样品的分离。样品的预处理多是先用甲醇浸提,然后用二氯甲烷-水进行液-液萃取,而目前SPE已成为继LLE之后的一个基本步骤。检测方法多采用紫外检测器,检测波长227~228nm。 *国家“八五”、“九五”重点科技攻关项目 作者单位:华中理工大学生物工程系 武汉430074
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In-Proc Natl Cancer Institute Workshop on Taxol and Taxus. 收稿日期:1998-10-05 |