高效液相色谱法测定石杉碱甲片的含量及其有关物质

摘　要：目的：采用高效液相色谱法测定石杉碱甲片的含量、含量均匀度以及有关物质。方法：采用ODS-C₁₈柱，以pH3.2磷酸三乙胺缓冲液-水(50∶50)为流动相，流速1mL^.min^-1，用紫外检测器于307nm波长处检测。结果：石杉碱甲在浓度为12.8～159.3μg^.mL^-1范围内，浓度与其峰面积呈良好的线性关系(r=0.99996)。平均回收率为100.3%，RSD=0.44%(n=21)。检出的最低量为石杉碱甲6ng。结论：较原试行标准简便，准确。

关键词：高效液相色谱法　石杉碱甲片　含量　含量均匀度　有关物质

分类号：R927　文献标识码：A

Assay of Content of Huperzine-A Tablets,Tests for Uniformity of Content and Its Related Substances by HPLC

WANG Guo-feng（Henan Drug Control Institute,Zhengzhou 450003）　
ZENG Fang（Henan Drug Control Institute,Zhengzhou 450003）　
ZHENG Zi-dong（Henan Drug Control Institute,Zhengzhou 450003）　

Abstract：Objective：To establish a HPLC method for the assay of the content of huperzine-A tablets,the uniformity of content and its related substances.Method:Using ODS-C₁₈ column,the mixture of buffer solution of phosphoric acid-triethylamine(pH 3.2)and water(50∶50)as mobile phase,at a flow rate of 1 mL^.min^-1,wavelength of the detector was 307 nm.Results:There is a good linear relationship within the range of 12.8～159.32 μg^.mL^-1(r=0.999 96).The average recovery is 100.3%，RSD is 0.44%(n=21).The lowest limit of detection is 6 ng.Conclusion:The new method is more rapid,simple and accurate than the old one.

Keywords：HPLC,huperzine-A tablets,content,uniformity of content,related substances

参考文献：

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收稿日期：2000年11月24日

出版日期：2001年3月31日