吡罗昔康凝胶有效成分的高效液相色谱研究

摘　要：本文提出了采用内标法，将HPLC用于吡罗昔康凝胶有效成分测定的新方法。讨论了流动相及内标的选择，进样量和空白基质的影响等。在优化的实验条件下，吡罗昔康浓度在0.02～0.2mg/mL范围内，其浓度与吡罗昔康和内标的峰面积之比呈线性关系。应用于实际样品分析，其结果与紫外分光光度法一致，平均回收率为99.6%，相对标准偏差为1.0%。方法简单,准确度高，专属性强，可用于吡罗昔康凝胶样品的稳定性考察和质量控制。

关键词：高效液相色谱；内标法；吡罗昔康；吡罗昔康凝胶

分类号：O657.72；R917　文献标识码：A
文章编号：1006-6144(2000)06-0470-04

A Study on the HPLC Method for the Determination of Piroxicam Gel

CHEN Hao（Huazhong Agricultural University, Wuhan, 430070）　
MI Zhi-yuan（Hubei Institute of Technology, Wuhan, 430068）　
LIU Wan-zhong（Hubei Provincial Institute for Drug Control, Wuhan, 430064）　

Abstract：In this paper, a new method for the determination of Piroxicam gel by the use of HPLC was reported. The main factors, including the selection of mobile phase and internal reference materials and effects of inject ion volume and matrix were investigated. Under optimum chromatographic condition s, the calibration graph was linear in the range of 0.02-0.2 mg/mL, the average recovery was 99.6% with a relative standard deviation of 1.0%. The method has be en applied to the determination of Piroxicam gel, and their results were in good agreement with those obtained by UV method. The proposed method was simple, acc urate, specific and applicable to the analysis of Piroxicam gel and quality cont rol.
Keywords：HPLC; Internal reference method; Piroxicam, Gelatum

参考文献：

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收稿日期：2000年7月11日

出版日期：2000年12月20日