摘 要:合成了3种纤维素衍生物并分别填充分析型色谱柱,研究了不同的涂敷条件对柱效的影响以及色谱柱的稳定性,对外消旋化合物进行了手性拆分。实现了高效液相色谱-旋光仪的联用,获得了在线旋光曲线。 关键词:手性固定相,拆分,高效液相色谱-旋光仪联用
分类号:O65 文献标识码:A
The Research of
Cellulose Derivatives as Chiral Stationary Phases and High Performance Liquid
Chromatography with Polarimeter as On-line Detector
Zhang Xin(Changchun
Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun
130022) Gao Lianxun(Changchun Institute of Applied Chemistry,Chinese Academy
of Sciences,Changchun 130022)
Abstract:Three cellulose derivatives were synthesized and used as chiral
stationary phases based on silica gel.The effects of adsorbances on the column
numbers and stabilities have been investigated.These stationary phases exhibited
high chiral recognition for various racemates.At the same time,the on-line
curves of polarimeter were obtained by high performance liquid chromatography
with polarimeter as on-line detector. Keywords:Chiral
stationary phase,cellulose derivatives,resolution,high performance liquid
chromatography polarimetry |
作者单位:张欣(中国科学院长春应用化学研究所,长春
130022)
高连勋(中国科学院长春应用化学研究所,长春 130022)
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收稿日期:2000年5月24日
修稿日期:2000年11月29日
出版日期:2001年5月20日
原载于《
分析化学》2001 Vol.29 No.5
P.526-529
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作者:
张欣高连勋 2007-5-18